||UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS
Conference: Bucharest University Faculty of Physics 2019 Meeting
Section: Biophysics; Medical Physics
Tumor spheroids for in vitro evaluation of antitumoral drugs
Mina RĂILEANU (1,2), Mihaela BACALUM (2), Aurel POPESCU (1)
1) University of Bucharest, Faculty of Physics
2) Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering
Doxorubicin, Gramicidin A, spheroids
Recently, the use of cell culture experiments with 3D spheroids reported results that could be better correlated with in vivo studies than the classical 2D cell culture systems. 3D spheroids can offer a simple and reproducible model that exhibit many characteristics of a natural tissue, such as the production of extracellular matrix [1,2,3].
In this study we used a colorectal adenocarcinom cell line (HT-29) that was screened and selected depending on their ability to form and maintain a spherical shape. We studied the effects of various concentrations of Doxorubicin and an antimicrobial peptide (Gramcidin A) on the integrity and viability of the selected spheroids which were then measured at regular intervals. Cytotoxicity of Doxorubicin and Gramcidin A occur very quickly after their administration, since the cellular activities and ATP production were decreasing after just 1 day of the treatment in all spheroids. The main effects of Doxorubicin and Gramicidin A towards the HT-29 spheroids also lead to degradation of the extracellular matrix, as all cells were dislodged from the spheroid upon cell death.
In conclusion, both Doxorubicin and Gramcidin A show high efficiency against the 3D system which will allow us to better understand the mechanism involved in the in vivo system.
1)Chism, D. D., De Silva, D. and Whang, Y. E. (2014) Mechanisms of acquired resistance to androgen receptor targeting drugs in castration-resistant prostate cancer, Expert. Rev. Anticancer Ther., 14, 1369-78.
2)Dutta, P. and Das, S. (2016) Mammalian Antimicrobial Peptides: Promising Therapeutic Targets Against Infection and Chronic Inflammation, Curr. Top. Med. Chem., 16, 99-129.
3)Fischbach, C., Chen, R., Matsumoto, T., Schmelzle, T., Brugge, J. S., Polverini, P. J. and Mooney, D. J. (2007) Engineering tumors with 3D scaffolds, Nat. Methods, 4, 855-60.
Mina Raileanu is preparing a PhD at Faculty of Physics, University of
Bucharest. This work was supported by the National Authority for
Research and Innovation (PN 18 09 02 02/2018, PN 19 06 02 03/2019,
PN-III-P1-1.2-PCCDI-2017-0010 / 74PCCDI ⁄ 2018)