2022-07-01 17:33


Conference: Bucharest University Faculty of Physics 2019 Meeting

Section: Physics and Technology of Renewable and Alternative Energy Sources

Biohybrids based on silver nanoparticles and standardized herbal extracts.

Cornelia NICHITA(1,2), Georgeta NEAGU(1), Theodor AXENIE (3), Sanda VOINEA(2), Faisal AL-BEHADILI(2), Mustafa ALHUSSAINY(2)

1)National Institute for Chemical – Pharmaceutical Research and Development, 112 Vitan Avenue, 031299, Bucharest, Romania

2)University of Bucharest, Faculty of Physics, 3Nanosae Research Center, MG-38, 405 Atomistilor Street, Bucharest-Magurele, Romania.

3) Department of Physics, Columbia University, 538 West 120th Street, 704 Pupin Hall, MC 5255, New York, NY, USA


herbal extracts, silver nanoparticles, bio-reduction method

Silver nanoparticles (AgNPs) have been exploited in a wide range of potential applications in medicine, cosmetics, renewable energies, environmental remediation and biomedical devices. The paper present the design, biosynthesis, physicochemical and biological properties of biohybrids based on silver nanoparticules and standardized plant extracts. The biohybrids based on silver nanoparticules enhanced stability, and also provide a means to deliver greater amounts of target therapeutic via high density surface coverage. They were used Ocimum basilicum L. standardized extracts (ObSE) containing flavonoids, polyphenols and phenolic carboxylic acids. This study reveals the relationship between silver nanoparticles and herbal extracts, and can lead to a new biohybrids with enhanced therapeutic anti inflammatory efficiency. To obtain the AgNPs, the bio-reduction method with the aqueous extract of Ocimum basilicum L. was applied. Selfassembling of the silver nanoparticles on ObSE for obtaing the biohybrids was performed by homogenization in the Ultra Turrax Digital High Speed system. The properties of the biohybrids were analyzed through various techniques: the presence and formation of AgNPs by UV-vis spectrometry, the particle size and zeta potential by dynamic light scattering (DLS). In addition the cytotoxicity of the biohybrids tested was determined in vitro using a mouse fibroblast culture line L929 (ATCC CRL-6364) and a human monocyte line SC (ATCC CRL 9855) as an experimental model. Evaluation of the anti-inflammatory effect in vitro was performed by assessing the production of nitric oxide in macrophages stimulated with LPS (lipopoly-saccharides).

This work was supported by the Romanian National Authority for Scientific Research, Project PCCDI/40/2018.