UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS

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2022-07-01 16:33

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Conference: Bucharest University Faculty of Physics 2019 Meeting


Section: Biophysics; Medical Physics


Title:
The spectroscopic study of the interaction of model lipid membranes with the genistein flavonoid


Authors:
Ioana Ileana FIDEL (1), Bogdan ZORILA (2), Claudia Gabriela CHILOM (1)


Affiliation:
1 Department of Electricity, Solid Physics and Biophysics, Faculty of Physics, University of Bucharest, Romania

2 Department of Life and Environmental Physics, “Horia Hulubei” National Institute of Physics and Nuclear Engineering, Măgurele, Romania



E-mail
fidel_ioana@yahoo.com


Keywords:
Liposomes, genistein, cholesterol, TMA-DPH, membrane stabilization


Abstract:
Flavonoids are active substances from plants that play an essential role in our lives (1). One of these flavonoids is genistein, a non-steroid extracted from soybean, which has numerous anti-cancer, antiangiogenic and antiatherogenic properties (2). Despite all the benefits it has on our health, genistein is not found in the body, but it has to be assimilated from different products. Once it gets in the body it should be transported to the place where its medical effects could be used. A simple way to transport genistein to the target site is to bind or embed it in liposomes. Due to their role as transporters, the liposomes could facilitate the delivery of genistein molecules at the required site in the body (3). In this paper we studied the interaction between two types of liposomes prepared from DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and DPPC:cholesterol (1,2-dipalmitoyl-sn-glycero-3-phosphocholine cholesterol), in a 75:25 molar ratio, and genistein. The time-resolved fluorescence and TMA-DPH fluorescence depolarization (1,6-diphenyl-1,3,5-hexatriene-4'-trimethylammonium tosylate) were used to characterize the samples. In the case of liposomes prepared from DPPC, the effect of genistein was manifested both in the fluorescence depolarization and time-resolved fluorescence spectra, with the life span being affected in the sense of decreasing with the increase in the genistein concentration. In the case of liposomes prepared from DPPC:cholesterol, genistein's presence did not have the same effect on the mean lifetime of fluorescence. This effect is due to the presence of cholesterol in the lipid bilayer. Thus, the cholesterol present in the lipid bilayer plays a role in stabilizing the bilayer and, at the same time, in the "isolation" of TMA-DPH to genistein.


References:

1. Panche N., Diwan A. D. and Chandra S. R., Flavonoids: an overview, Journal of Nutritional Science, 5, e47, 1-15, 2016.

2. Polkowski K. and Mazurek A. P., Biological properties of genistein. A review of in vitro and in vivo data, Acta Pol Pharm., 57(2):135-55, 2000.

3. Bożena P. P., Misiak L. E., Zarzyka B., Paduch R., Gawron A. and Gruszecki W. I., Localization and interaction of genistein with model membranes formed with dipalmitoylphosphatidylcholine (DPPC), Biochimica et Biophysica Acta, 1818, 1785–1793, 2012



Acknowledgement:
This work was supported by the Romanian Ministry of Education and Research through Grant: PN 19 06 02 03